In Tiny Toon Adventures, Wile E. Coyote is the dean of Acme Looniversity and the mentor of Calamity Coyote. The Road Runner's protégé in this series is Little Beeper.

Early model sheets for the character prior to his initial appearance in Fast and Furry-ous identified him as "Don Coyote", a pun of the name Don Quixote.

Tech E. Coyote is the tech expert of the Loonatics (based on Wile E. Coyote's interest in Acme gadgets), with magnetic hands and the ability to molecularly regenerate himself (based on the many instances in which Wile E. miraculously recovered after plummeting to his death).