Uncle Ben first appeared in Amazing Fantasy #15 (August 1962) and was killed in the very same issue. Although his history as a supporting character was very brief, the character is an overshadowing figure in Spider-Man's life and often appears in flashbacks.

Jameson would typically accept Bannon's photos because they made Spider-Man look bad.

After Iron Man convinced him to reveal his identity to the public, Peter worried so much about his decision that he threw up in the White House bathroom.

J. Jonah Jameson's son, John Jameson, was an astronaut selected for a secret mission to the moon. While collecting lunar rock samples, he came across a glittering red gemstone which, after his return to Earth, would later graft itself to his skin, causing him to transform into a wolf like humanoid creature known as Man-Wolf when exposed to the light of a full moon.

In Amazing Spider-Man #3, Spidey was badly beaten in his first-ever encounter with Doctor Octupus. Mentally defeated, Peter questioned his ability to go on as Spider-Man until he attended a school assembly where the Human Torch was giving a speech. The Torch encouraged the students to "never give up" even when things seemed tough. Peter took this message to heart, and in a rematch, defeated Doctor Octopus handily.

Betty Brant first appeared in Amazing Spider-Man #4, working for the Daily Bugle as J. Jonah Jameson's secretary. Years later (Amazing Spider-Man #142), Peter would tell Mary Jane that Betty was his first love.

Felicia Hardy (a.k.a. the Black Cat)competed against Mary Jane Watson for Peter's affections, but eventually lost out. She also dated Flash Thompson.

In Amazing Spider-Man #16, Spider-Man and Daredevil battled the Ringmaster and his Circus of Crime for the very first time.

Mysterio (Quentin Beck) started his career as a Hollywood stuntman. He later branched into special effects but quickly grew tried of his constant backstage role, and when a friend jokingly suggested that, if he really wanted to become famous, he should take out one of the new costumed crime-fighters, Beck decided to kill Spider-Man, thus launching his criminal career.

The Amazing Spider-Man, featuring Nicholas Hammond as Spider-Man, aired in the United States from September 19, 1977 to July 6, 1979. Although it was a considerable ratings success, the CBS series was cancelled after just 13 episodes.